Studying melanin and lipofuscin in RPE cell culture models

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dc.contributor.authorBoulton, Michael E
dc.contributor.departmentDepartment of Ophthalmology, IU School of Medicineen_US
dc.date.accessioned2016-01-22T16:45:45Z
dc.date.available2016-01-22T16:45:45Z
dc.date.issued2014-09
dc.description.abstractThe retinal pigment epithelium contains three major types of pigment granules; melanosomes, lipofuscin and melanolipofuscin. Melanosomes in the retinal pigment epithelium (RPE) are formed during embryogenesis and mature during early postnatal life while lipofuscin and melanolipofuscin granules accumulate as a function of age. The difficulty in studying the formation and consequences of melanosomes and lipofuscin granules in RPE cell culture is compounded by the fact that these pigment granules do not normally occur in established RPE cell lines and pigment granules are rapidly lost in adult human primary culture. This review will consider options available for overcoming these limitations and permitting the study of melanosomes and lipofuscin in cell culture and will briefly evaluate the advantages and disadvantages of the different protocols.en_US
dc.eprint.versionAuthor's manuscripten_US
dc.identifier.citationBoulton, M. E. (2014). Studying melanin and lipofuscin in RPE cell culture models. Experimental Eye Research, 126, 61–67. http://doi.org/10.1016/j.exer.2014.01.016en_US
dc.identifier.issn0014-4835en_US
dc.identifier.urihttps://hdl.handle.net/1805/8155
dc.language.isoen_USen_US
dc.publisherElsevieren_US
dc.relation.isversionof10.1016/j.exer.2014.01.016en_US
dc.relation.journalExperimental eye researchen_US
dc.rightsPublisher Policyen_US
dc.sourcePMCen_US
dc.subjectCells, Cultureden_US
dc.subjectcytologyen_US
dc.subjectLipofuscinen_US
dc.subjectphysiologyen_US
dc.subjectEpithelial Cellsen_US
dc.subjectMelaninsen_US
dc.subjectRetinal Pigment Epitheliumen_US
dc.titleStudying melanin and lipofuscin in RPE cell culture modelsen_US
dc.typeArticleen_US
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