De Novo Sequencing of Peptides from High-Resolution Bottom-Up Tandem Mass Spectra using Top-Down Intended Methods
dc.contributor.author | Vyatkina, Kira | |
dc.contributor.author | Dekker, Lennard J. M. | |
dc.contributor.author | Wu, Si | |
dc.contributor.author | VanDuijn, Martijn M. | |
dc.contributor.author | Liu, Xiaowen | |
dc.contributor.author | Tolić, Nikola | |
dc.contributor.author | Luider, Theo M. | |
dc.contributor.author | Paša-Tolić, Ljiljana | |
dc.contributor.department | BioHealth Informatics, School of Informatics and Computing | en_US |
dc.date.accessioned | 2017-12-21T18:55:06Z | |
dc.date.available | 2017-12-21T18:55:06Z | |
dc.date.issued | 2017-12 | |
dc.description.abstract | Despite high-resolution mass spectrometers are becoming accessible for more and more laboratories, tandem (MS/MS) mass spectra are still often collected at a low resolution. And even if acquired at a high resolution, software tools used for their processing do not tend to benefit from that in full, and an ability to specify a relative mass tolerance in this case often remains the only feature the respective algorithms take advantage of. We argue that a more efficient way to analyze high-resolution MS/MS spectra should be with methods more explicitly accounting for the precision level, and sustain this claim through demonstrating that a de novo sequencing framework originally developed for (high-resolution) top-down MS/MS data is perfectly suitable for processing high-resolution bottom-up datasets, even though a top-down like deconvolution performed as the first step will leave in many spectra at most a few peaks. | en_US |
dc.eprint.version | Author's manuscript | en_US |
dc.identifier.citation | Vyatkina, K., Dekker, L. J. M., Wu, S., VanDuijn, M. M., Liu, X., Tolić, N., Luider, T. M. and Paša-Tolić, L. (2017), De Novo Sequencing of Peptides from High-Resolution Bottom-Up Tandem Mass Spectra using Top-Down Intended Methods. Proteomics, 17(23-24), 1600321. http://dx.doi.org/10.1002/pmic.201600321 | en_US |
dc.identifier.uri | https://hdl.handle.net/1805/14873 | |
dc.language.iso | en | en_US |
dc.publisher | Wiley | en_US |
dc.relation.isversionof | 10.1002/pmic.201600321 | en_US |
dc.relation.journal | Proteomics | en_US |
dc.rights | Publisher Policy | en_US |
dc.source | Author | en_US |
dc.subject | bottom-up proteomics | en_US |
dc.subject | high-resolution mass spectrometry | en_US |
dc.subject | de novo sequencing | en_US |
dc.title | De Novo Sequencing of Peptides from High-Resolution Bottom-Up Tandem Mass Spectra using Top-Down Intended Methods | en_US |
dc.type | Article | en_US |