A VP22-Null HSV-1 Is Impaired in Inhibiting CD1d-Mediated Antigen Presentation
dc.contributor.author | Liu, Jianyun | |
dc.contributor.author | Gallo, Richard M. | |
dc.contributor.author | Duffy, Carol | |
dc.contributor.author | Brutkiewicz, Randy R. | |
dc.contributor.department | Microbiology and Immunology, School of Medicine | en_US |
dc.date.accessioned | 2018-03-06T21:38:02Z | |
dc.date.available | 2018-03-06T21:38:02Z | |
dc.date.issued | 2016-09-01 | |
dc.description.abstract | CD1d-restricted T (natural killer T [NKT]) cells are important for controlling a herpes simplex virus (HSV) infection. One of the mechanisms of immune evasion by HSV is to downregulate CD1d-mediated activation of NKT cells. VP22 is an HSV-1-encoded protein responsible for reorganizing the host cell's cytoskeletal network and viral spreading. We have previously shown that modification of the cytoskeleton can alter CD1d-mediated antigen presentation. In this study, we found that an HSV-1 lacking VP22 (ΔUL49) was impaired in its ability to inhibit CD1d-mediated antigen presentation compared with the wild-type (WT) virus; this was reversed by a repair virus (UL49R) in CD1d-expressing cells. We further demonstrated that CD1d recycling was inhibited by infection with WT and UL49R, but not the ΔUL49 virus. Ectopic expression of VP22 in CD1d-expressing cells complemented the VP22-deficient virus in inhibiting antigen presentation. Moreover, inhibiting viral protein synthesis rescued VP22-dependent inhibition of CD1d antigen presentation. In conclusion, our findings suggest that VP22 is required (but not sufficient) for the inhibition of CD1d-mediated antigen presentation by an HSV-1 infection. | en_US |
dc.eprint.version | Final published version | en_US |
dc.identifier.citation | Liu, J., Gallo, R. M., Duffy, C., & Brutkiewicz, R. R. (2016). A VP22-Null HSV-1 Is Impaired in Inhibiting CD1d-Mediated Antigen Presentation. Viral Immunology, 29(7), 409–416. https://doi.org/10.1089/vim.2015.0145 | en_US |
dc.identifier.issn | 0882-8245 | en_US |
dc.identifier.uri | https://hdl.handle.net/1805/15382 | |
dc.language.iso | en_US | en_US |
dc.publisher | Mary Ann Leibert | en_US |
dc.relation.isversionof | 10.1089/vim.2015.0145 | en_US |
dc.relation.journal | Viral Immunology | en_US |
dc.rights | Publisher Policy | en_US |
dc.source | PMC | en_US |
dc.subject | HSV | en_US |
dc.subject | CD1d-restricted T cells | en_US |
dc.title | A VP22-Null HSV-1 Is Impaired in Inhibiting CD1d-Mediated Antigen Presentation | en_US |
dc.type | Article | en_US |
ul.alternative.fulltext | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5031099/ | en_US |