MiR-9 Controls Chemotactic Activity of Cord Blood CD34⁺ Cells by Repressing CXCR4 Expression
dc.contributor.author | Ha, Tae Won | |
dc.contributor.author | Kang, Hyun Soo | |
dc.contributor.author | Kim, Tae-Hee | |
dc.contributor.author | Kwon, Ji Hyun | |
dc.contributor.author | Kim, Hyun Kyu | |
dc.contributor.author | Ryu, Aeli | |
dc.contributor.author | Jeon, Hyeji | |
dc.contributor.author | Han, Jaeseok | |
dc.contributor.author | Broxmeyer, Hal E. | |
dc.contributor.author | Hwang, Yongsung | |
dc.contributor.author | Lee, Yun Kyung | |
dc.contributor.author | Lee, Man Ryul | |
dc.contributor.department | Microbiology and Immunology, School of Medicine | en_US |
dc.date.accessioned | 2019-06-05T19:26:51Z | |
dc.date.available | 2019-06-05T19:26:51Z | |
dc.date.issued | 2018-11-30 | |
dc.description.abstract | Improved approaches for promoting umbilical cord blood (CB) hematopoietic stem cell (HSC) homing are clinically important to enhance engraftment of CB-HSCs. Clinical transplantation of CB-HSCs is used to treat a wide range of disorders. However, an improved understanding of HSC chemotaxis is needed for facilitation of the engraftment process. We found that ectopic overexpression of miR-9 and antisense-miR-9 respectively down- and up-regulated C-X-C chemokine receptor type 4 (CXCR4) expression in CB-CD34+ cells as well as in 293T and TF-1 cell lines. Since CXCR4 is a specific receptor for the stromal cell derived factor-1 (SDF-1) chemotactic factor, we investigated whether sense miR-9 and antisense miR-9 influenced CXCR4-mediated chemotactic mobility of primary CB CD34+ cells and TF-1 cells. Ectopic overexpression of sense miR-9 and antisense miR-9 respectively down- and up-regulated SDF-1-mediated chemotactic cell mobility. To our knowledge, this study is the first to report that miR-9 may play a role in regulating CXCR4 expression and SDF-1-mediated chemotactic activity of CB CD34+ cells. | en_US |
dc.identifier.citation | Ha, T. W., Kang, H. S., Kim, T. H., Kwon, J. H., Kim, H. K., Ryu, A., … Lee, M. R. (). MiR-9 Controls Chemotactic Activity of Cord Blood CD34⁺ Cells by Repressing CXCR4 Expression. International journal of stem cells, 11(2), 187–195. doi:10.15283/ijsc18057 | en_US |
dc.identifier.uri | https://hdl.handle.net/1805/19535 | |
dc.language.iso | en_US | en_US |
dc.publisher | Korean Society for Stem Cell Research | en_US |
dc.relation.isversionof | 10.15283/ijsc18057 | en_US |
dc.relation.journal | International Journal of Stem Cells | en_US |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 United States | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/us/ | * |
dc.source | PMC | en_US |
dc.subject | CXCR4 | en_US |
dc.subject | Cell migration | en_US |
dc.subject | Cord blood | en_US |
dc.subject | microRNA | en_US |
dc.title | MiR-9 Controls Chemotactic Activity of Cord Blood CD34⁺ Cells by Repressing CXCR4 Expression | en_US |
dc.type | Article | en_US |