Monitoring focal adhesion kinase phosphorylation dynamics in live cells

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dc.contributor.authorDamayanti, Nur P.
dc.contributor.authorBuno, Kevin
dc.contributor.authorNarayanan, Nagarajan
dc.contributor.authorHarbin, Sherry L Voytik
dc.contributor.authorDeng, Meng
dc.contributor.authorIrudayaraj, Joseph M.K.
dc.contributor.departmentMedicine, School of Medicineen_US
dc.date.accessioned2019-05-09T19:45:10Z
dc.date.available2019-05-09T19:45:10Z
dc.date.issued2017-07-24
dc.description.abstractFocal adhesion kinase (FAK) is a cytoplasmic non-receptor tyrosine kinase essential for a diverse set of cellular functions. Current methods for monitoring FAK activity in response to an extracellular stimulus lack spatiotemporal resolution and/or the ability to perform multiplex detection. Here we report on a novel approach to monitor the real-time kinase phosphorylation activity of FAK in live single cells by fluorescence lifetime imaging.en_US
dc.eprint.versionAuthor's manuscripten_US
dc.identifier.citationDamayanti, N. P., Buno, K., Narayanan, N., Voytik Harbin, S. L., Deng, M., & Irudayaraj, J. (2017). Monitoring focal adhesion kinase phosphorylation dynamics in live cells. The Analyst, 142(15), 2713–2716. doi:10.1039/c7an00471ken_US
dc.identifier.urihttps://hdl.handle.net/1805/19203
dc.language.isoen_USen_US
dc.publisherRoyal Society of Chemistryen_US
dc.relation.isversionof10.1039/c7an00471ken_US
dc.relation.journalThe Analysten_US
dc.rightsPublisher Policyen_US
dc.sourcePMCen_US
dc.subjectBiosensing Techniquesen_US
dc.subjectCells, Cultureden_US
dc.subjectFluorescenceen_US
dc.subjectFocal Adhesion Protein-Tyrosine Kinasesen_US
dc.subjectMicroscopy, Confocalen_US
dc.subjectPhosphorylationen_US
dc.subjectSingle-Cell Analysisen_US
dc.subjectTyrosineen_US
dc.titleMonitoring focal adhesion kinase phosphorylation dynamics in live cellsen_US
dc.typeArticleen_US
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