• Kinetic Analysis of Vasculogenesis Quantifies Dynamics of Vasculogenesis and Angiogenesis In Vitro 

  Varberg, Kaela M.; Winfree, Seth; Dunn, Kenneth W.; Haneline, Laura S. (JoVE, 2018-01-31)

  Vasculogenesis is a complex process by which endothelial stem and progenitor cells undergo de novo vessel formation. Quantitative assessment of vasculogenesis has become a central readout of endothelial progenitor cell ...
• Loss of the protective arm of renin-angiotensin-system results in hematopoietic stem / progenitor cell defects, gut dysbiosis and retinopathy in diabetes 

  Duan, Yaqian (2017-12-11)

  Angiotensin-converting enzyme 2 (ACE2) is the primary enzyme of the vasoprotective axis of the renin-angiotensin system (RAS). We tested the hypothesis that the protective arm of the RAS axis can i) act to maintain homeostasis ...
• Endothelial colony-forming cells ameliorate endothelial dysfunction via secreted factors following ischemia-reperfusion injury 

  Collett, Jason A.; Mehrotra, Purvi; Crone, Allison; Shelley, W. Christopher; Yoder, Mervin C.; Basile, David P. (American Physiological Society, 2017-05-01)

  Damage to endothelial cells contributes to acute kidney injury (AKI) by leading to impaired perfusion. Endothelial colony-forming cells (ECFC) are endothelial precursor cells with high proliferative capacity, pro-angiogenic ...
• Exocytosis proteins as novel targets for diabetes prevention and/or remediation? 

  Aslamy, Arianne; Thurmond, Debbie C. (American Physiological Society, 2017-05-01)

  Diabetes remains one of the leading causes of morbidity and mortality worldwide, affecting an estimated 422 million adults. In the US, it is predicted that one in every three children born as of 2000 will suffer from ...
• Pancreatic ductal adenocarcinoma cell secreted extracellular vesicles containing ceramide-1-phosphate promote pancreatic cancer stem cell motility 

  Kuc, Norbert; Doermann, Allison; Shirey, Carolyn; Lee, Daniel D.; Lowe, Chinn-Woan; Awasthi, Niranjan; Schwarz, Roderich E.; Stahelin, Robert V.; Schwarz, Margaret A. (Elsevier, 2018-10)

  The high mortality rate associated with pancreatic ductal adenocarcinoma (PDAC) is in part due to lack of effective therapy for this highly chemoresistant tumor. Cancer stem cells, a subset of cancer cells responsible for ...
• Cardiovascular consequences of metabolic syndrome 

  Tune, Johnathan D.; Goodwill, Adam G.; Sassoon, Daniel J.; Mather, Kieren J. (Elsevier, 2017-05)

  The metabolic syndrome (MetS) is defined as the concurrence of obesity-associated cardiovascular risk factors including abdominal obesity, impaired glucose tolerance, hypertriglyceridemia, decreased HDL cholesterol, and/or ...
• Epicardial Adipose Tissue Removal Potentiates Outward Remodeling and Arrests Coronary Atherogenesis 

  McKenney-Drake, Mikaela L.; Rodenbeck, Stacey D.; Bruning, Rebecca S.; Kole, Ayeeshik; Yancey, Kyle W.; Alloosh, Mouhamad; Sacks, Harold S.; Sturek, Michael (Elsevier, 2017-05)

  BACKGROUND: Pericoronary epicardial adipose tissue (cEAT) serves as a metabolic and paracrine organ that contributes to inflammation and is associated with macrovascular coronary artery disease (CAD) development. Although ...
• Regulation of Coronary Blood Flow 

  Goodwill, Adam G.; Dick, Gregory M.; Kiel, Alexander M.; Tune, Johnathan D. (Wiley, 2017-03-16)

  The heart is uniquely responsible for providing its own blood supply through the coronary circulation. Regulation of coronary blood flow is quite complex and, after over 100 years of dedicated research, is understood to ...
• Argonaute 2 Expression Correlates with a Luminal B Breast Cancer Subtype and Induces Estrogen Receptor Alpha Isoform Variation 

  Conger, Adrienne K.; Martin, Elizabeth C.; Yan, Thomas J.; Rhodes, Lyndsay V.; Hoang, Van T.; La, Jacqueline; Anbalagan, Muralidharan; Burks, Hope E.; Rowan, Brian G.; Nephew, Kenneth P.; Collins-Burow, Bridgette M.; Burow, Matthew E. (MDPI, 2016-09-21)

  Estrogen receptor alpha (ERα) signaling pathways are frequently disrupted in breast cancer and contribute to disease progression. ERα signaling is multifaceted and many ERα regulators have been identified including ...
• Kinetic analyses of vasculogenesis inform mechanistic studies 

  Varberg, Kaela M.; Winfree, Seth; Chu, Chenghao; Tu, Wanzhu; Blue, Emily K.; Gohn, Cassandra R.; Dunn, Kenneth W.; Haneline, Laura S. (American Physiological Society, 2017-04-01)

  Vasculogenesis is a complex process by which endothelial stem and progenitor cells undergo de novo vessel formation. Quantitative assessment of vasculogenesis is a central readout of endothelial progenitor cell functionality. ...
• Strengths and Weaknesses of Recently Engineered Red Fluorescent Proteins Evaluated in Live Cells Using Fluorescence Correlation Spectroscopy 

  Siegel, Amanda P.; Baird, Michelle A.; Davidson, Michael W.; Day, Richard N. (MDPI, 2013-10-14)

  The scientific community is still looking for a bright, stable red fluorescent protein (FP) as functional as the current best derivatives of green fluorescent protein (GFP). The red FPs exploit the reduced background of ...
• Monitoring Biosensor Activity in Living Cells with Fluorescence Lifetime Imaging Microscopy 

  Hum, Julia M.; Siegel, Amanda P.; Pavalko, Fredrick M.; Day, Richard N. (MDPI, 2012-11-07)

  Live-cell microscopy is now routinely used to monitor the activities of the genetically encoded biosensor proteins that are designed to directly measure specific cell signaling events inside cells, tissues, or organisms. ...
• PKC-dependent Phosphorylation of the H1 Histamine Receptor Modulates TRPC6 Activity 

  Chen, Xingjuan; Egly, Christian; Riley, Ashley M.; Li, Wennan; Tewson, Paul; Hughes, Thomas E.; Quinn, Anne Marie; Obukhov, Alexander G. (MDPI, 2014-04-04)

  Transient receptor potential canonical 6 (TRPC6) is a cation selective, DAG-regulated, Ca2+-permeable channel activated by the agonists of Gq-protein-coupled heptahelical receptors. Dysfunctions of TRPC6 are implicated in ...
• Fast assessment of lipid content in arteries in vivo by intravascular photoacoustic tomography 

  Cao, Yingchun; Kole, Ayeeshik; Hui, Jie; Zhang, Yi; Mai, Jieying; Alloosh, Mouhamad; Sturek, Michael; Cheng, Ji-Xin (Nature Publishing Group, 2018-02-05)

  Intravascular photoacoustic tomography is an emerging technology for mapping lipid deposition within an arterial wall for the investigation of the vulnerability of atherosclerotic plaques to rupture. By converting localized ...
• The P2Y2 nucleotide receptor is an inhibitor of vascular calcification 

  Qian, Shaomin; Regan, Jenna N.; Shelton, Maxwell T.; Hoggatt, April; Mohammad, Khalid S.; Herring, Paul B.; Seye, Cheikh I. (Elsevier, 2017-02)

  BACKGROUND AND AIMS: Mutations in the 5'-nucleotidase ecto (NT5E) gene that encodes CD73, a nucleotidase that converts AMP to adenosine, are linked to arterial calcification. However, the role of purinergic receptor ...
• New aspects of cellular cholesterol regulation on blood glucose control- review and perspective on the impact of statin medications on metabolic health 

  Grice, Brian A.; Elmendorf, Jeffrey S. (2017-01-01)

  Cholesterol is an essential component of cell membranes, and during the past several years, diabetes researchers have found that membrane cholesterol levels in adipocytes, skeletal muscle fibers and pancreatic beta cells ...
• Metabolism of hydrogen sulfide (H2S) and Production of Reactive Sulfur Species (RSS) by superoxide dismutase 

  Olson, Kenneth R.; Gao, Yan; Arif, Faihaan; Arora, Kanika; Patel, Shivali; DeLeon, Eric. R.; Sutton, Thomas R.; Feelisch, Martin; Cortese-Krott, Miriam M.; Straub, Karl D. (Elsevier, 2017-11-20)

  Reactive sulfur species (RSS) such as H2S, HS•, H2Sn, (n = 2–7) and HS2•- are chemically similar to H2O and the reactive oxygen species (ROS) HO•, H2O2, O2•- and act on common biological effectors. RSS were present in ...
• Endothelial Cell-Specific Deletion of P2Y2 Receptor Promotes Plaque Stability in Atherosclerosis-Susceptible ApoE-Null Mice 

  Xingjuan, Chen; Qian, Shaomin; Hoggatt, April; Tang, Hongying; Hacker, Timothy A.; Obukhov, Alexander G.; Herring, Paul B.; Seye, Cheikh I. (American Heart Association, 2017-01)

  OBJECTIVE: Nucleotide P2Y2 receptor (P2Y2R) contributes to vascular inflammation by increasing vascular cell adhesion molecule-1 expression in endothelial cells (EC), and global P2Y2R deficiency prevents fatty streak ...
• Molecular Determinants of the Sensitivity to Gq/11-Phospholipase C-dependent Gating, Gd3+ Potentiation, and Ca2+ Permeability in the Transient Receptor Potential Canonical Type 5 (TRPC5) Channel 

  Chen, Xingjuan; Li, Wennan; Riley, Ashley M.; Soliman, Mario; Chakraborty, Saikat Chakrabort; Stamatkin, Christopher W.; Obukhov, Alexander G. (American Society for Biochemistry and Molecular Biology, 2017-01-20)

  Transient receptor potential canonical type 5 (TRPC5) is a Ca2+-permeable cation channel that is highly expressed in the brain and is implicated in motor coordination, innate fear behavior, and seizure genesis. The channel ...
• Rho Kinase (ROCK) collaborates with Pak to Regulate Actin Polymerization and Contraction in Airway Smooth Muscle 

  Zhang, Wenwu; Bhetwal, Bhupal P.; Gunst, Susan J. (Wiley, 2018)

  Rho kinase (ROCK), a RhoA GTPase effector, can regulate the contraction of airway and other smooth muscle tissues. In some tissues, ROCK can inhibit myosin regulatory light chain (RLC) phosphatase, which increases the ...

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