• Recent advances in bio-orthogonal and dynamic crosslinking of biomimetic hydrogels 

  Arkenberg, Matthew R.; Nguyen, Han D.; Lin, Chien-Chi (Royal Society of Chemistry, 2020-09-21)

  In recent years, dynamic, 'click' hydrogels have been applied in numerous biomedical applications. Owing to the mild, cytocompatible, and highly specific reaction kinetics, a multitude of orthogonal handles have been ...
• Mentor-focused Professional Development for Investigators Initiating Discipline-based Educational Research (DBER) in Biomedical Engineering 

  Miller, Sharon; Higbee, Steven (ASEE, 2021-07)

  Our work (NSF PFE: RIEF Award 1927150) initiates a discipline-based educational research study of student design self-efficacy in an undergraduate biomedical engineering (BME) program. A key component of this work focuses ...
• Generation of the tumor-suppressive secretome from tumor cells 

  Liu, Shengzhi; Sun, Xun; Li, Kexin; Zha, Rongrong; Feng, Yan; Sano, Tomohiko; Dong, Chuanpeng; Liu, Yunlong; Aryal, Uma K.; Sudo, Akihiro; Li, Bai-Yan; Yokota, Hiroki (Ivyspring International, 2021-07-25)

  Rationale: The progression of cancer cells depends on the soil and building an inhibitory soil might be a therapeutic option. We previously created tumor-suppressive secretomes by activating Wnt signaling in MSCs. Here, ...
• Morphological and mechanical characterization of bone phenotypes in the Amish G610C murine model of osteogenesis imperfecta 

  Kohler, Rachel; Tastad, Carli A.; Creecy, Amy; Wallace, Joseph M. (PLOS, 2021-08-27)

  Osteogenesis imperfecta (OI) is a hereditary bone disease where gene mutations affect Type I collagen formation resulting in osteopenia and increased fracture risk. There are several established mouse models of OI, but ...
• Polyurethane coated with polyvinylpyrrolidones via triazole links for enhanced surface fouling resistance 

  Wen, Xin; Almousa, Rashed; Na, Sungsoo; Anderson, Gregory G.; Xie, Dong (Wiley, 2021-12)

  Surfaces with hydrophilic and antimicrobial properties are very attractive for cardiovascular device-associated applications. The aim of this study was to prepare and coat a hydrophilic polymer containing a functional group ...
• Overexpression of Lrp5 enhanced the anti-breast cancer effects of osteocytes in bone 

  Liu, Shengzhi; Wu, Di; Sun, Xun; Fan, Yao; Zha, Rongrong; Jalali, Aydin; Feng, Yan; Li, Kexin; Sano, Tomohiko; Vike, Nicole; Li, Fangjia; Rispoli, Joseph; Sudo, Akihiro; Liu, Jing; Robling, Alexander; Nakshatri, Harikrishna; Li, Bai-Yan; Yokota, Hiroki (Springer Nature, 2021-07-06)

  Osteocytes are the most abundant cells in bone, which is a frequent site of breast cancer metastasis. Here, we focused on Wnt signaling and evaluated tumor-osteocyte interactions. In animal experiments, mammary tumor cells ...
• A Reversible Low Frequency Alternating Current Nerve Conduction Block Applied to Mammalian Autonomic Nerves 

  Muzquiz, M. Ivette; Mintch, Landan; Horn, M. Ryne; Alhawwash, Awadh; Bashirullah, Rizwan; Carr, Michael; Schild, John H.; Yoshida, Ken (MDPI, 2021-07)

  Electrical stimulation can be used to modulate activity within the nervous system in one of two modes: (1) Activation, where activity is added to the neural signalling pathways, or (2) Block, where activity in the nerve ...
• Mechanical tibial loading remotely suppresses brain tumors by dopamine-mediated downregulation of CCN4 

  Fan, Yao; Zha, Rongrong; Sano, Tomohiko; Zhao, Xinyu; Liu, Shengzhi; Woollam, Mark D.; Wu, Di; Sun, Xun; Li, Kexin; Egi, Motoki; Li, Fangjia; Minami, Kazumasa; Siegel, Amanda P.; Horiuchi, Takashi; Liu, Jing; Agarwal, Mangilal; Sudo, Akihiro; Nakshatri, Harikrishna; Li, Bai-Yan; Yokota, Hiroki (Springer Nature, 2021-05-24)

  Mechanical loading to the bone is known to be beneficial for bone homeostasis and for suppressing tumor-induced osteolysis in the loaded bone. However, whether loading to a weight-bearing hind limb can inhibit distant tumor ...
• In-vivo application of low frequency alternating currents on porcine cervical vagus nerve evokes reversible nerve conduction block 

  Muzquiz, Maria Ivette; Richardson, Lindsay; Vetter, Christian; Smolik, Macallister; Alhawwash, Awadh; Goodwill, Adam; Bashirullah, Rizwan; Carr, Michael; Yoshida, Ken (BMC, 2021-06-30)

  Background: This paper describes a method to reversibly block nerve conduction through direct application of a 1 Hz sinusoidal current waveform delivered through a bipolar nerve cuff electrode. This low frequency alternating ...
• Tumor Cell Secretomes in Response to Anti- and Pro-Tumorigenic Agents 

  Liu, Sheng-Zhi; Sun, Xun; Li, Ke-Xin; Lin, Chien-Chi; Na, Sungsoo; Li, Bai-Yan; Yokota, Hiroki (MDPI, 2021)

  Tumor cells regulate their progression not only by the factors within their cell bodies but also by the secretome they produce and secrete. While their secretome significantly alters the fate of tumor cells themselves, ...
• Finite Element Analysis as an Iterative Design Tool for Students in an Introductory Biomechanics Course 

  Higbee, Steven; Miller, Sharon (ASME, 2021-12)

  Insufficient engineering analysis is a common weakness of student capstone design projects. Efforts made earlier in a curriculum to introduce analysis techniques should improve student confidence in applying these important ...
• Preventing tumor progression to the bone by induced tumor-suppressing MSCs 

  Sun, Xun; Li, Kexin; Zha, Rongrong; Liu, Shengzhi; Fan, Yao; Wu, Di; Hase, Misato; Aryal, Uma K.; Lin, Chien-Chi; Li, Bai-Yan; Yokota, Hiroki (Ivyspring International, 2021-03-05)

  Background: Advanced breast cancer metastasizes to many organs including bone, but few effective treatments are available. Here we report that induced tumor-suppressing (iTS) MSCs protected bone from metastases while ...
• The Effect of Single Versus Group μCT on the Detection of Trabecular and Cortical Disease Phenotypes in Mouse Bones 

  Kohler, Rachel; Tastad, Carli A.; Stacy, Alexander J.; Swallow, Elizabeth A.; Metzger, Corinne E.; Allen, Matthew R.; Wallace, Joseph M. (Wiley, 2021-03-05)

  Micro‐computed tomography is a critical assessment tool for bone‐related preclinical research, especially in murine models. To expedite the scanning process, researchers often image multiple bones simultaneously; however, ...
• Engineering Tools for Regulating Hypoxia in Tumour Models 

  Kim, Min Hee; Green, Steven D.; Lin, Chien-Chi; Konig, Heiko (Wiley, 2021)

  Major advances in the field of genomic technologies have led to an improvement in cancer diagnosis, classification and prognostication. However, many cancers remain incurable due to the development of drug resistance, ...
• Hydrogel Models with Stiffness Gradients for Interrogating Pancreatic Cancer Cell Fate 

  Chang, Chun-Yi; Lin, Chien-Chi (MDPI, 2021-03)

  Pancreatic ductal adenocarcinoma (PDAC) is the most common type of pancreatic cancer and has seen only modest improvements in patient survival rate over the past few decades. PDAC is highly aggressive and resistant to ...
• Hydrogel Models with Stiffness Gradients for Interrogating Pancreatic Cancer Cell Fate 

  Chang, Chun-Yi; Lin, Chien-Chi (MDPI, 2021-03-13)

  Pancreatic ductal adenocarcinoma (PDAC) is the most common type of pancreatic cancer and has seen only modest improvements in patient survival rate over the past few decades. PDAC is highly aggressive and resistant to ...
• Inhibition of the Growth of Breast Cancer-Associated Brain Tumors by the Osteocyte-Derived Conditioned Medium 

  Sano, Tomohiko; Sun, Xun; Feng, Yan; Liu, Shengzhi; Hase, Misato; Fan, Yao; Zha, Rongrong; Wu, Di; Aryal, Uma K.; Li, Bai-Yan; Sudo, Akihiro; Yokota, Hiroki (MDPI, 2021-03-03)

  The brain is a common site of metastasis from advanced breast cancer but few effective treatments are available. We examined a therapeutic option with a conditioned medium (CM), focusing on the role of Lrp5 and β-catenin ...
• Knee loading repairs osteoporotic osteoarthritis by relieving abnormal remodeling of subchondral bone via Wnt/β-catenin signaling 

  Zheng, Weiwei; Ding, Beibei; Li, Xinle; Liu, Daquan; Yokota, Hiroki; Zhang, Ping (Wiley, 2020-02)

  Osteoporotic osteoarthritis (OPOA) is a common bone disease mostly in the elderly, but the relationship between Osteoporotic (OP) and osteoarthritis (OA) is complex. It has been shown that knee loading can mitigate OA ...
• Suppression of sost/sclerostin and dikkopf-1 augment intervertebral disc structure in mice 

  Kroon, Tori; Bhadouria, Neharika; Niziolek, Paul; Edwards, Daniel; Clinkenbeard, Erica L.; Robling, Alexander; Holguin, Nilsson (Wiley, 2022)

  Intervertebral disc (IVD) degeneration is a leading cause of low back pain, characterized by accelerated extracellular matrix breakdown and IVD height loss but there is no approved pharmacological therapeutic. Deletion of ...
• Loading-induced antitumor capability of murine and human urine 

  Wu, Di; Fan, Yao; Liu, Shengzhi; Woollam, Mark D.; Sun, Xun; Murao, Eiji; Zha, Rongrong; Prakash, Rahul; Park, Charles; Siegel, Amanda P.; Liu, Jing; Agarwal, Mangilal; Li, Bai-Yan; Yokota, Hiroki (Wiley, 2020-06)

  While urine has been considered as a useful bio-fluid for health monitoring, its dynamic changes to physical activity are not well understood. We examined urine's possible antitumor capability in response to medium-level, ...

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