Monitoring focal adhesion kinase phosphorylation dynamics in live cells
Date
2017-07-24
Language
American English
Embargo Lift Date
Department
Committee Members
Degree
Degree Year
Department
Grantor
Journal Title
Journal ISSN
Volume Title
Found At
Royal Society of Chemistry
Abstract
Focal adhesion kinase (FAK) is a cytoplasmic non-receptor tyrosine kinase essential for a diverse set of cellular functions. Current methods for monitoring FAK activity in response to an extracellular stimulus lack spatiotemporal resolution and/or the ability to perform multiplex detection. Here we report on a novel approach to monitor the real-time kinase phosphorylation activity of FAK in live single cells by fluorescence lifetime imaging.
Description
item.page.description.tableofcontents
item.page.relation.haspart
Cite As
Damayanti, N. P., Buno, K., Narayanan, N., Voytik Harbin, S. L., Deng, M., & Irudayaraj, J. (2017). Monitoring focal adhesion kinase phosphorylation dynamics in live cells. The Analyst, 142(15), 2713–2716. doi:10.1039/c7an00471k
ISSN
Publisher
Series/Report
Sponsorship
Major
Extent
Identifier
Relation
Journal
The Analyst
Rights
Publisher Policy
Source
PMC
Alternative Title
Type
Article
Number
Volume
Conference Dates
Conference Host
Conference Location
Conference Name
Conference Panel
Conference Secretariat Location
Permanent Link
Version
Author's manuscript