Zhang, HongjiVoytik-Harbin, SherryBrookes, SarahZhang, LujuanWallace, JosephParker, NoahHalum, Stacey2019-09-032019-09-032018-04Zhang, H., Voytik-Harbin, S., Brookes, S., Zhang, L., Wallace, J., Parker, N., & Halum, S. (2018). Use of autologous adipose-derived mesenchymal stem cells for creation of laryngeal cartilage. The Laryngoscope, 128(4), E123–E129. doi:10.1002/lary.26980https://hdl.handle.net/1805/20732OBJECTIVES/HYPOTHESIS: Adipose-derived mesenchymal stem cells (ASCs) are an exciting potential cell source for tissue engineering because cells can be derived from the simple excision of autologous fat. This study introduces a novel approach for tissue-engineering cartilage from ASCs and a customized collagen oligomer solution, and demonstrates that the resultant cartilage can be used for laryngeal cartilage reconstruction in an animal model. STUDY DESIGN: Basic science experimental design. METHODS: ASCs were isolated from F344 rats, seeded in a customized collagen matrix, and cultured in chondrogenic differentiation medium for 1, 2, and 4 weeks until demonstrating cartilage-like characteristics in vitro. Large laryngeal cartilage defects were created in the F344 rat model, with the engineered cartilage used to replace the cartilage defects, and the rats followed for 1 to 3 months. Staining examined cellular morphology and cartilage-specific features. RESULTS: In vitro histological staining revealed rounded chondrocyte-appearing cells evenly residing throughout the customized collagen scaffold, with positive staining for cartilage-specific markers. The cartilage was used to successfully repair large cartilaginous defects in the rat model, with excellent functional results. CONCLUSIONS: This study is the first study to demonstrate, in an animal model, that ASCs cultured in a unique form of collagen oligomer can create functional cartilage-like grafts that can be successfully used for partial laryngeal cartilage replacement.en-USPublisher PolicyLarynxCartilageTissue engineeringCollagenOligomerChondrocytesAdipose stem cellArticle