Relich, R. F.Schmitt, B. H.Raposo, H.Barker, L.Blosser, S. J.May, M.2018-08-292018-08-292018-04-01Relich, R. F., Schmitt, B. H., Raposo, H., Barker, L., Blosser, S. J., & May, M. (2018). Legionella indianapolisensis sp. nov., isolated from a patient with pulmonary abscess. International Journal of Infectious Diseases, 69, 26–28. https://doi.org/10.1016/j.ijid.2018.01.0241201-9712https://hdl.handle.net/1805/17245Background To date, at least 50 species of Legionella have been described. These organisms are ubiquitous in nature and have been isolated from diverse ecological environments, including man-made structures such as cooling towers and spas. Legionellae have also been isolated from human and veterinary clinical specimens, and their roles in disease are well-established. This report describes the isolation of a novel Legionella species from a respiratory specimen from a patient with influenza and suspected pulmonary embolus. Case A 68-year-old male presented to an Indianapolis-area hospital with pulmonary disease; upon workup, he was found to have influenza A. Bronchoalveolar lavage fluid was also submitted for conventional bacterial culture and Legionella culture. The patient was prescribed a broad-spectrum antibiotic and recovered. Results A Legionella-like bacterium was isolated on buffered charcoal yeast extract agar, and mass spectrometry and comparative 16S rRNA gene sequencing inconclusively identified the isolate as a Legionella sp. Further analysis of the 16S rRNA gene confirmed the strain to be a new species, related to Legionella hackeliae. Physiochemical and morphological testing were used to confirm the discovery of a novel species, Legionella indianapolisensis sp. nov., type strain SMNF-IS.en-USAttribution-NonCommercial-NoDerivs 3.0 United StatesLegionella indianapolisensisLegionellaLegionellaePulmonary abscessInfluenzaCo-infectionLobar pneumoniaArticle