Sohutskay, David O.Buno, Kevin P.Tholpady, Sunil S.Nier, Samantha J.Voytik-Harbin, Sherry L.2022-06-212022-06-212020-02Sohutskay DO, Buno KP, Tholpady SS, Nier SJ, Voytik-Harbin SL. Design and biofabrication of dermal regeneration scaffolds: role of oligomeric collagen fibril density and architecture. Regen Med. 2020;15(2):1295-1312. doi:10.2217/rme-2019-0084https://hdl.handle.net/1805/29384Aim: To evaluate dermal regeneration scaffolds custom-fabricated from fibril-forming oligomeric collagen where the total content and spatial gradient of collagen fibrils was specified. Materials & methods: Microstructural and mechanical features were verified by electron microscopy and tensile testing. The ability of dermal scaffolds to induce regeneration of rat full-thickness skin wounds was determined and compared with no fill control, autograft skin and a commercial collagen dressing. Results: Increasing fibril content of oligomer scaffolds inhibited wound contraction and decreased myofibroblast marker expression. Cellular and vascular infiltration of scaffolds over the 14-day period varied with the graded density and orientation of fibrils. Conclusion: Fibril content, spatial gradient and orientation are important collagen scaffold design considerations for promoting vascularization and dermal regeneration while reducing wound contraction.en-USPublisher PolicyCustom fabricationMechanobiologyOligomeric collagenRegenerationSkin and wound careArticle