Lautscham, Lena A.Lin, Corey Y.Auernheimer, VeraNaumann, Christoph A.Goldmann, Wolfgang H.Fabry, Ben2016-03-022016-03-022014-03Lautscham, L. A., Lin, C. Y., Auernheimer, V., Naumann, C., Goldmann, W. H., & Fabry, B. (2014). Biomembrane-mimicking lipid bilayer system as a mechanically tunable cell substrate. Biomaterials, 35(10), 3198–3207. http://doi.org/10.1016/j.biomaterials.2013.12.091 Download as: RIShttps://hdl.handle.net/1805/8633Cell behavior such as cell adhesion, spreading, and contraction critically depends on the elastic properties of the extracellular matrix. It is not known, however, how cells respond to viscoelastic or plastic material properties that more closely resemble the mechanical environment that cells encounter in the body. In this report, we employ viscoelastic and plastic biomembrane-mimicking cell substrates. The compliance of the substrates can be tuned by increasing the number of polymer-tethered bilayers. This leaves the density and conformation of adhesive ligands on the top bilayer unaltered. We then observe the response of fibroblasts to these property changes. For comparison, we also study the cells on soft polyacrylamide and hard glass surfaces. Cell morphology, motility, cell stiffness, contractile forces and adhesive contact size all decrease on more compliant matrices but are less sensitive to changes in matrix dissipative properties. These data suggest that cells are able to feel and respond predominantly to the effective matrix compliance, which arises as a combination of substrate and adhesive ligand mechanical properties.en-USPublisher PolicyMechanosensingBiomimetic materialsPhospholipid bilayer stacksComplianceArticle