Scherschel, John A.Soonpaa, Mark H.Srour, Edward F.Field, Loren J.Rubart, Michael2020-12-092020-12-092008-06-01Scherschel, J. A., Soonpaa, M. H., Srour, E. F., Field, L. J., & Rubart, M. (2008). Adult bone marrow–derived cells do not acquire functional attributes of cardiomyocytes when transplanted into peri-infarct myocardium. Molecular Therapy, 16(6), 1129-1137.https://hdl.handle.net/1805/24556(BM) cells after being directly transplanted into the ischemically injured heart remains a controversial issue. In this study, we investigated the ability of transplanted BM cells to develop intracellular calcium ([Ca2+] i ) transients in response to membrane depolarization in situ. Low-density mononuclear (LDM) BM cells, c-kit-enriched (c-kitenr) BM cells, and highly enriched lin– c-kit+ BM cells were obtained from adult transgenic mice ubiquitously expressing enhanced green fluorescent protein (EGFP), and injected into peri-infarct myocardiums of nontransgenic mice. After 9–10 days the mice were killed, and the hearts were removed, perfused in Langendorff mode, loaded with the calcium-sensitive fluorophore rhod-2, and subjected to two-photon laser scanning fluorescence microscopy (TPLSM) to monitor action potential–induced [Ca2+] i transients in EGFP-expressing donor-derived cells and non-expressing host cardiomyocytes. Whereas spontaneous and electrically evoked [Ca2+] i transients were found to occur synchronously in host cardiomyocytes along the graft–host border and in areas remote from the infarct, they were absent in all of the >3,000 imaged BM-derived cells that were located in clusters throughout the infarct scar or peri-infarct zone. We conclude that engrafted BM-derived cells lack attributes of functioning cardiomyocytes, calling into question the concept that adult BM cells can give rise to substantive cardiomyocyte regeneration within the infarcted heart.en-USAttribution-NonCommercial-NoDerivatives 4.0 InternationalBone MarrowCellsFunctional AttributesCardiomyocytesPeri-infarct MyocardiumArticle