Rajaram, KrithikaGiebel, Amanda M.Toh, EvelynHu, ShuaiNewman, Jasmine H.Morrison, Sandra G.Kari, LaszloMorrison, Richard P.Nelson, David E.2015-07-212015-07-212015-07Rajaram, K., Giebel, A. M., Toh, E., Hu, S., Newman, J. H., Morrison, S. G., ... & Nelson, D. E. (2015). Mutational analysis of the Chlamydia muridarum plasticity zone. Infection and immunity, IAI-00106.https://hdl.handle.net/1805/6575Pathogenically diverse Chlamydia spp. can have surprisingly similar genomes. C. trachomatis isolates that cause trachoma, sexually transmitted genital tract infections (chlamydia) and invasive lymphogranuloma venereum (LGV), and the murine strain C. muridarum share 99% of their gene content. A region of high genomic diversity between Chlamydia spp. termed the Plasticity Zone (PZ) may encode niche-specific virulence determinants that dictate pathogenic diversity. We hypothesized that PZ genes might mediate the greater virulence and IFN-γ resistance of C. muridarum compared to C. trachomatis in the murine genital tract. To test this hypothesis, we isolated and characterized a series of C. muridarum PZ nonsense mutants. Strains with nonsense mutations in chlamydial cytotoxins, guaBA-add and a phospholipase D homolog developed normally in cell culture. Two of the cytotoxin mutants were less cytotoxic than wild-type suggesting that the cytotoxins may be functional. However, none of the PZ nonsense mutants exhibited increased IFN-γ sensitivity in cell culture or were profoundly attenuated in a murine genital tract infection model. Our results suggest that C. muridarum PZ genes are transcribed and some may produce functional proteins, but are dispensable for infection of the murine genital tract.en-USchlamydiamutational analysisplasticity zoneArticle